بررسی سازوکار مولکولی فعال‌سازی سامانه ایمنی گاو با استفاده از داده‌های بیان دیجیتالی لوکوسیت‌ها

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی مقطع دکتری، گروه علوم دامی، دانشگده علوم دامی و صنایع غذایی، دانشگاه کشاورزی و منابع طبیعی رامین-خوزستان

2 استاد، گروه علوم دامی، دانشگده علوم دامی و صنایع غذایی، دانشگاه کشاورزی و منابع طبیعی رامین-خوزستان

3 استادیار، گروه علوم دامی، دانشکده کشاورزی و منابع طبیعی، دانشگاه یاسوج

4 استادیار، گروه علوم دامی، دانشگده علوم دامی و صنایع غذایی، دانشگاه کشاورزی و منابع طبیعی رامین-خوزستان

5 استادیار، گروه دام و طیور، پردیس ابوریحان، دانشگاه تهران

چکیده

کاربرد مسیرهای ژنی مؤثر در بروز صفات مختلف در اصلاح نژاد به راحتی امکان‌پذیر نمی‌باشد، اما شناسایی مسیرهای ژنی بدست آمده از برهم کنش داده‌های ترانسکریپتومی تأثیرگذار بر یک فرآیند زیستی، می‌تواند گام مهمی در فهم بهتر سازوکارهای تنظیمی آن فرآیند باشد. در این پژوهش، چگونگی سازوکار مولکولی تقویت پاسخ ایمنی با در نظر گرفتن مسیرهای ژنی فعال شده در لوکوسیت‌ها، مورد بررسی قرار گرفت. برای رسیدن به این هدف، پس از پیاده‌سازی داده‌های حاصل از تکنیک توالی‌یابی رونوشت‌ها (RNA-Seq) با شماره دسترسی GSE37447 از پایگاه داده NCBI، کنترل کیفیت داده‌ها با نرم‌افزار FastQC بررسی گردید. مکان‌یابی ژن‌ها و ایزوفرم‌ها با استفاده از نرم افزار TopHat2 انجام شد و تعیین میزان بیان هر ژن و ایزوفرم با نرم‌افراز HT-Seq انجام گرفت و نهایتاً بررسی ژن‌های متفاوت بیان شده با edgeR انجام گرفت. آنالیز مسیرهای ژنی بر اساس ژن‌هایی که تفاوت بیان داشتند، با پایگاه اطلاعاتی DAVID صورت گرفت. آنالیز مسیرهای ژنی نشان داد، مسیرهای اسپلایسوزم و ترمیم اتصالات نادرست ژنوم در لوکوسیت‌ها فعال می‌شود. بنابراین، مسیرهای ژنی یاد شده می‌توانند چگونگی تقویت پاسخ ایمنی را تفسیر نمایند و به روشن شدن بهتر مسیرهای تنظیمی در این فرآیند بیولوژیکی کمک کنند. به نظر می‌رسد جهت بهبود سامانه ایمنی به کمک برنامه‌های اصلاح نژادی می‌توان از ژن‌ها، فاکتورهای رونویسی، میکرو ریبونوکلئیک اسیدها و یا کوفاکتورهای دخیل در این دو مسیر ژنی (مسیر ژنی اتصال جایگزین و مسیر ترمیم اتصالات نادرست) کمک گرفت، زیرا این دو مسیر ژنی به طور مستقیم در فعال‌سازی و تنظیم فعالیت سامانه ایمنی مؤثر هستند.

کلیدواژه‌ها

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