Genetic structure analysis of partial promoter region of Lactophorin gene in Iranian Dromedary and Bactrian camels

Document Type : Research Paper

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Abstract

Camel milk is regarded as one of the richest protein sources. Lactophorin is one of the most important proteins in camel milk whey. Promoter Regions are those which have special significance in gene expression .The aim of this study was examined the structure of promoter region of Lactophorin gene with about 790 nucleotide in two Iranian Dromedary and Bactrian camels. 10 and 5 blood samples were obtained from Dromedary and Bactrian camels, respectively. The considered region was amplified and sequenced by using of specific primers. lactophorin gene structure was investigated by using genetic analysis. After aligning of the obtained gene sequences, 4 haplotype in dromedary, and 3 haplotype in Bactrian camels were observed. In addition, 12 and 10 motifs were found in dromedary and Bactrian camels, respectively. Consequently, regarding the fact that there is no information about the structure and sequencing of camel milk protein especially in Iranian species, it seems that doing some complementary studies due to specifying and analyzing the gene structure of this protein may provide more comprehensive and beneficial information about the changes and probable variations of the gene in Iranian species.

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References

Agrawal, R.P., Swami, S.C., Beniwal, R., Kochar, D.K., Sahani, M.S., Tuteja, F.C. and Ghouri, S.K. (2003). Effect of camel milk on glycemic control, risk factors and diabetes quality of life in type-1 diabetes: a randomised prospective controlled study. Journal of Camel Practice and Research. 10(1), 45-50.
Beg, O.U., Von Bahr-Lindström, H., Zaidi, Z.H. and Jörnvall, H. (1987). Characterization of a heterogeneous camel milk whey non-casein protein. FEBS Letters. 216(2), 270-274.
Baumhueter, S., Mendel, D.B., Conley, P.B., Kuo, C.J., Turk, C., Graves, M.K., Edwards, C. A., Courtois, G. and Crabtree, G.R. (1989). HNF-1  shares  three  sequence  motifs  with the  POU  domain  proteins  and  is  identical to  LF-B1  and  APF. Genes and Development. 4(3), 372-379.
Courtois, G., Morgan, J.G., Campbell, L.A., Fourel, G. and Crabtree, G.R. (1987). Interaction  of  a  liver-specific  nuclear  factor with  the  fibrinogen  and alpha 1-antitrypsin  promoters. Science. 238(4827), 688-692.
Courtois, G., Baumhueter, S. and Crabtree, G.R. (1988). Purified hepatocyte nuclear factor 1 interacts with a family of hepa- tocyte-specific promoters. Proceedings of the National Academy of Sciences of the United States of America. 85(21), 7937-7941.
ELagamy, E.I., Nawar, M., Shamsia, S.M., Awad, S. and Haenlein, G.F.W. (2009). Are Camel Milk Proteins Convenient to the Nutrition of Cow Milk Allergic Children? .Small Ruminant Research. 82:1-6.
Fucharoen, S., Shimizu, K. and Fukumaki, Y. (1990). A novel C–T transition within the distal CCAAT motif of the gamma-globin gene in the Japanese HPFH: Implication of factor binding in elevated fetal globin expression. Nucleic Acids Research. 18, 52-45.
Grontved, L., Mandrup, S. (2009). Mus muscle is peroxisome proliferator activated receptor Gamma. Transcription Factor Encyclopedia.
Gu, M., Liu, Q., Watanabe, S.H., Sun, L., Hollopeter, G., Grant, B.D. and Jorgensen, E.M. (2013). AP2 hemicomplexes contribute independently to synaptic vesicle endocytosis. E life. 2(00190).  
Johnsen, L.B., Petersen, T.E. and Berglund, L. (1996). The bovine PP3 gene is homologous to the murine GlyCAM-1 gene. Gene. 169: 297-298.
Kappeler, S., Heuberger, C., Farah, Z. and Puhan, Z. (2004). Expression of the peptidoglycan recognition protein, PGRP, in the lactating mammary gland. Journla of Dairy Science. 87(8), 2660-2668.
 Kappeler, S., Farah, Z. and Puhan, Z. (1991). Alternative Splicing of Lactophorin mRNA from Lactating Mammary Gland of the Camel (Camelus dromedarius). Journal of Dairy Science. 82:2084–2093.
 Kenzhebulat, S., Ermuhan, B. and Tleuov, A. (2000). Composition of camel milk and its use in the treatment of infectious diseases in human. In: Proceedings of the 2nd Camelid Conference on Agroeconomics of Camelid Farming, Almaty, Kazakhstan, September 8–12, AgroMerkur Publication,, 101.
 Lum, L.S., Dovc, P. and Medrano, J.F. (1997). Polymorphisms of bovine beta-lactoglobulin promoter and differences in the binding affinity of activator protein-2 transcription factor. Journal of Dairy Science. 80(7), 1389-1397.
 Lai, E., Prezioso, V.R., Tao, W., Chen, W. S. and Darnell, J.E. (1991). Hepatocyte nuclear factor 3 alpha belongs to a gene family in mammals that is homologous to the Drosophila homeotic gene fork head. Genes and Development. 5: 416–427.
Magjeed, N.A. (2005). Corrective effect of milk camel on some cancer biomarkers in blood of rats intoxicated with aflatoxin B1. Journal of Saudi Chemical society. 9(2), 253–263.
 Malewski, T. (1998). Computer analysis of distribution of putative cis- and Trans regulatory elements in milk protein gene promoters. Biosystems. 45(1), 29-44.
 Szymanowska, M., Malewski, T. and Zwierzchowski, L. (2003). Transcription factor binding to variable nucleotide sequences in 5′-flanking regions of bovine casein genes. International Dairy Journal. 14(2), 103-115.
 Pjanic, M., Schmid, C.D., Gaussin, A., Ambrosini, G., Adamcik, J., Pjanic, P., Plasari, G., Kerschgens, J., Dietler, D., Bucher, P.H., Mermod, N. and Mermod, N. (2013). Nuclear Factor I genomic binding associates with chromatin boundaries. BMC Genomics. 14(99), 1471-2164.
 Shabo, Y., Barzel, R., Margoulis, M. and Yagil, R. (2005). The Israel Medical Association Journal. Immunology and Allergy. 7(12), 796-798.
 Stefan, K. (1998). Compositional and structural analysis of camel milk proteins with emphasis on protective proteins. Diss. ETH No. 12947.
Watson, C., Gordon, K., Robertson, M. and Clark, J. (1991). Interaction of DNA-binding proteins with a milk protein Gene promoter in vitro: identification of a mammary gland-specific factor. Nucleic Acids Research. 19(23), 6603-6610.
 Wedel, A. and Ziegler-Heitbrock, H.W. (1995). The C/EBP Family of Transcription Factors. Immunobiology. 193(2-4), 171-185.
 Food and Agriculture Organization (2000). Biodiversity: Agricultural biodiversity in FAO. Retrieved January 12, 2009, from http://www.fao.org/biodiversity.
Animal Sciences Journal
Volume 29, Issue 113
March 2017
Pages 77-86

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