Determination of chemical composition and nutritive value of radish leaf in ruminant feeding in vitro

Document Type : Research Paper

Authors

1 Department of Animal Science, School of Agriculture, Lorestan University

2 Lorestan University

Abstract

This experiment was conducted to determine chemical composition and nutritive value of radish leaf (RL) in vitro. In the first stage, chemical composition of radish leaves were determined, then its fermentation parameters and nutrients disappearance was investigated using in vitro gas production (GP) and two-stage nutrient digestion methods compared to wheat straw (WS) and alfalfa. In the second stage, RL was included in the diet at the levels of 0, 50, 100, 150 or 200 g/kg dry matter (DM) and incubated in vitro. Results showed that DM, organic matter (OM), neutral detergent fibre (NDF), acid detergent fibre (ADF), lignin, Ca and P content of RL were 135, 752, 178, 385, 281, 52, 17.5 and 2.85 g/kg DM, respectively. Total volume of gas production and nutrient disappearance were significantly higher in RL compared to WS (p < 0.05), and most of these parameters were comparable with alfalfa. Including different levels of RL in the diet increased DM and OM disappearance (p < 0.05). Highest and lowest short chain fatty acids, and two-stage DM and NDF disappearance were observed by incubation of diet containing 200 g RL and control diet, respectively (p < 0.05). Overall, results of the present study showed that radish leaf has better nutritive value than that of WS, and it is comparable with alfalfa in term of rumen digestion of fermentation. Inclusion of this by-product in the diet up to 200 g/kg DM is recommended.

Keywords

References

آمارنامه کشاورزی. 1395. وزارت جهاد کشاورزی. جلد دوم. 401 ص.
بیضایی، س.، صفی پور افشار، الف. و سعید نعمت پور، ف. (1393). تولید ترکیبات فنولی در کشت ریشه های مویین گیاه تربچه. مجله پژوهشهای سلولی مولکولی (مجله زیست شناسی ایران). 28 (3): 327-335.
تیمورنژاد، ن.، زاهدی‌فر، م.، نیکخواه، ع. و فضائلی، ح. (1385). تعیین ارزش غذایی پس مانده‌های میوه و سبزیجات در نشخوارکنندگان. نشریه پژوهش و سازندگی (امور دام و آبزیان). 76: 173-168.
AOAC. (1990). Official methods of analysis of the Association of official Analytical chemists. Edited by kenneth Helrich. 15th edition. USA.
Arias, L., Contreras, J., Losada, H., Grande, D., Soriano, R., Vieyra, J., Cortés, J. and Rivera, J.  (2003). A note on the chemical composition and in vitro digestibility of common vegetables utilised in urban dairy systems of the east of Mexico City. Livestock Research for Rural Development 15 (2): 1-6.
Azizi, A., Maia, M.R.G., Fonseca, A.J.M., Sharifi, A., Fazaeli, H. and Cabrita, A.R.J. (2018). Rumen fermentation of lignocellulosic biomass from wheat straw and date leaf inoculated with bacteria isolated from termite gut. Journal of Animal and Feed Science. 27: 211-218.
Azizi-Shotorkhoft, A., Rezaei, J. and Fazaeli, H. (2013). The effect of different levels of molasses on the digestibility, rumen parameters and blood metabolites in sheep fed processed broiler litter. Animal Feed Science and Technology. 179: 69–76.
Azizi-Shotorkhoft, A., Rouzbehan, Y. and Fazaeli H. (2012). The influence of the different carbohydrate sources on utilization efficiency of processed broiler litter in sheep. Livestock Science. 148: 249–254.
Bhatnagar, M.K., Azhar, B.M. 2016. A study of chemical composition of some leafy vegetables of fatehpur district. International Journal of Science and Research, 5, 408-410.
Blümmel, M., Steingss, H. and Becker, K. (1997). The relationship between in vitro gas production, in vitro microbial biomass yield and 15N incorporation and its implications for the prediction of voluntary feed intake of roughages. British Journal of Nutrition. 77: 911–921.
Broderick, G. and Kang, J.H. (1980). Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. Journal of Dairy Science. 63: 64–75.
Chamberlain, D.G., Robertson, S. and Choung, J.J. (1993). Sugars versus starch as supplements to grass silage: effects on ruminal fermentation and the supply of microbial protein to the small intestine, estimated from the urinary excretion of purine derivatives in sheep. Journal of Science of Food and Agricultur, 63: 189–194.
FAO. (2013). Utilization of fruit and vegetable wastes as livestock feed and as substrates for generation of other value-added products. RAP Publication, Rome, Italy.
Fazaeli, H. and Frough Ameri, N.(2006). Utilization of pistachio by product in the diet of finishing calves. Proceeding of the EAAP annual meeting, Antalya, Turkey. C33: 51p.
Getachew, G., Makkar, H.P.S. and Becker, K. (2002). Tropical browses: contents of phenolic compounds, in vitro gas production and stoichiometric relationship between short chain fatty acid and in vitro gas production. Journal of Agricultural Science. 139: 341–352.
Jeon, S., Sohn, K.N. and Seo, S. (2016). Evaluation of feed value of a by-product of pickled radish for ruminants: analyses of nutrient composition, storage stability, and in vitro ruminal fermentation. Journal of Animal Science and Technology, 58: 1-9.
Marten, G.C. and Barnes, R.F. (1980). Prediction of energy digestibility of forages with in vitro rumen fermentation and fungal enzymes systems. In: Pidgen, W. J., Balch, C. C. & Graham, M. (Eds), Standardization of analytical methodology for feeds. (pp 61-71.) International Development Research Center, Ottawa.
Menke, K.H. and Steingass, H. (1988). Estimation of the energetic feed value obtained from chemical analysis and gas production using rumen fluid. Animal Research and Development. 28: 7–55.
NRC, (2007). National Research Council, Nutrient requirements of small ruminants: Sheep, Goats, Cervids, and New World Camelids. Washington (DC, USA): National Academy of Sciences.
SAS, (2005). User’s Guide: Statistics, Version 9.0 Edition. SAS Inst. Inc., Cary, NC.
Tilley, J.M.A. and Terry, R.A. (1963). A two stage technique for the indigestion of forage crops. Journal of the British Grass-land Society. 18, 104-111.
Van Soest, P.J. (1994). Nutritional Ecology of the Ruminant, 2nd ed. Cornell Univ. Press, Itacha, NY, USA, 476 pp.
Van Soest, P.J., Robertson, J.B. and Lewis, B.A. (1991). Methods for dietary fiber, neutral detergent fiber and non starch polysaccharides in relation to animal nutrition. Journal of Dairy Science. 74: 3583–3597.
Vercoe, P. E., Makkar, H.P.S. and Schlink, A.C. (2010). In vitro screening of plant resources for extra-nutritional attributes in ruminants: nuclear and related methodologies. Springer Verlag Gmbh.
Wadhwa, M. and Bakshi, M.P.S. ( 2005). Vegetable wastes − A potential source of nutrients for ruminants. Indian Journal of Animal Nutrition, 22: 70−76.
Animal Sciences Journal
Volume 33, Issue 129
March 2021
Pages 49-60

Files

Share

How to cite

Statistics