اثرات افزودن پودر خرفه(Portulace oleracea L.) با ویتامین E و ال –کارنیتین در جیره کم پروتئین بر ایمنی و فراسنجه‌های خونی در جوجه‌های گوشتی تحت تنش سرمایی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری تغذیه طیور، گروه علوم دامی، دانشگاه کردستان

2 استاد گروه علوم دامی، دانشگاه کردستان

چکیده

آزمایش حاضر برای بررسی اثرات افزودن پودر خرفه، ویتامین E و ال-کارنیتین در جیره‌های کم پروتئین بر سیستم ایمنی و فراسنجه‌های بیوشیمیایی خون در جوجه‌های گوشتی پرورش یافته در شرایط استرس سرمایی انجام شد. 576 جوجه گوشتی نر یک روزه (راس 308) به طور تصادفی به 6 گروه آزمایشی تقسیم شد که هر گروه آزمایشی دارای 6 تکرار و 16 پرنده در هر تکرار بود. گروه‌های آزمایشی شامل: (1) جیره با پروتئین معمول (شاهد منفی)، (2) جیره کم پروتئین (2 درصد کمتر نسبت به شاهد منفی) به عنوان شاهد، (3) جیره شاهد + 2 گرم بر کیلوگرم پودر خرفه، (4) جیره شاهد حاوی پودر خرفه + 250 میلی گرم برکیلوگرم ویتامین E ، (5) جیره شاهد حاوی پودر خرفه + 150 میلی گرم برکیلوگرم ال-کارنیتین و (6) جیره شاهد حاوی پودر خرفه + مخلوط ویتامین E و ال-کارنیتین بودند. نتایج نشان داد که افزودن پودر خرفه + ویتامین E و پودر خرفه + ال-کارنیتین به جیره شاهد شمار گلبول‌های قرمز خون، هموگلوبین، هماتوکریت و نسبت هتروفیل به لنفوسیت را در 42 روزگی کاهش دادند (05/0< P). تیتر آنتی بادی علیه گلبول‌های قرمز گوسفندی و ویروس بیماری نیوکاسل به ترتیب در روزهای 41 و 27 توسط ویتامین E و ال-کارنیتین افزایش یافت(05/0< P)، در حالی که میزان تری‌گلیسرید و کلسترول نیز توسط آن‌ها کاهش پیدا کرد(05/0< P). افزودن ویتامین E و ال-کارنیتین به تنهایی در ترکیب با خرفه در جیره کم پروتئین با ایجاد اثرات سینرژیک می‌تواند موجب بهبود سیستم ایمنی و سلامت در جوجه‌های گوشتی تحت استرس سرمایی ‌شود.

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