Effects of medicinal plant Silybum marianum on core diameter and DNA fragmentation in the sperm of rams Farahani treated with cadmium

Document Type : Research Paper

Authors

1 Department of Biology

2 Department of Animal Science Faculty of Agriculture Arak university

3 Department of biology faculty of Science Arak university

Abstract

The sperm used in the current study was collected from the testis epidydim of slaughtered Farahani's ram and divided to four groups. There are 106 sperms in each sample. The experimental treatments included sperm at 0 hour, sperm at 180 minutes (control), sperm treated with cadmium chloride (10μM) for 180 minutes and sperm treated with silymarin (5 μM) and cadmium chloride (10 μM) for 180 minutes. Diff-Quick staining was used to investigate the apoptosis morphological features in sperm nucleus. Also, acridine orange staining and sperm chromatin dispersion (SCD) test were used for estimation of sperm DNA fragmentation. Data were analyzed using one way ANOVA. Comparison of treatment means was done by Tukey's test at 0.05 confidence level.In this research, the sperm nucleus diameter was significantly (P< 0.05) decreased in cadmium chloride-treated group when compared with control. The utilization of silymarin with cadmium chloride could compensate significantly the reduction in sperm nucleus diameter in cadmium chloride- treated group. The results also showed that the DNA fragmentation percentage was significantly increased in cadmium chloride- treated group in comparison to control (P<0.001) and silymarin with cadmium chloride-treated group (P< 0.01). Silymarin as a potent antioxidant can inhibit the detrimental effect of cadmium chloride on ram sperm nucleus diameter and DNA integrity.

Keywords

References

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Animal Sciences Journal
Volume 30, Issue 117
March 2018
Pages 215-226

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